Twinning in matching black-and-white striped Pjs and polka-dot socks, the sisters sang the classic lullaby while someone played the piano.
“A little night Music,” the Holiday hitmaker, 58, captioned the video, alongside a series of star emojis.
Earlier this month, the Material Mom announced her adoption joy.
“I can officially confirm I have completed the process of adopting twin sisters from Malawi and am overjoyed that they are now part of our family,” she captioned image walking hand-in-hand with her daughters.
She added: “I am deeply grateful to all those in Malawi who helped make this possible, and I ask the media please to respect our privacy during this transitional time. Thank you also to my friends, family and my very large team for all your support and Love!”
The mom-of-six adopted son David and daughter Mercy James, both 11, also from Malawi in 2008 and 2009, respectively.
The Papa Don’t Preach singer – who is also mom to daughter Lourdes, 20, and 16-year-old son Rocco – founded her Raising Malawi foundation in 2006 to benefit the orphans and underprivileged children living in the impoverished nation.
The singer adopted David with ex-husband Guy Ritchie, and adopted Mercy James as a single mother.