“On my way to Jimmy Kimmel!! Make sure you tune in tonight! AND (obviously), DON’T MISS THE SUPERNATURAL SEASON 13 PREMIERE!! #spnfamily #akf #supernatural,” he captioned the image toting his tot.
Last month, the proud papa shared a selfie with his baby girl.
In August, the Gilmore Girls star, 35, and his wife, Wildfire actress Genevieve Cortese Padalecki, 36, were all smiles with their 3-year-old son Shepherd in a family selfie.
The couple – who are also parents to 5-year-old son Thomas – welcomed their daughter on March 17.
The father-of-three recently penned a heartfelt letter to his sons and daughter.